Abstract
A Pseudomonas fluorescens isolate (PF-1) resistant to carbapenems was recovered during an environmental survey performed with water from the Seine River (Paris). It expressed a novel Ambler class A carbapenemase, BIC-1, sharing 68 and 59% amino acid identities with beta-lactamases SFC-1 from Serratia fonticola and the plasmid-encoded KPC-2, respectively. beta-Lactamase BIC-1 hydrolyzed penicillins, carbapenems, and cephalosporins except ceftazidime and monobactams. The bla(BIC-1) gene was chromosomally located and was also identified in two other P. fluorescens strains isolated from the Seine River 3 months later.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / metabolism
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Bacterial Proteins / genetics
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Blotting, Southern
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Cloning, Molecular
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial / genetics
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Electrophoresis, Gel, Pulsed-Field
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France
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Fresh Water / microbiology
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Gram-Negative Bacteria / drug effects
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Health Surveys
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Kinetics
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Plasmids / genetics
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Pseudomonas fluorescens / enzymology*
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Water Microbiology
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beta-Lactamases / chemistry
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA, Bacterial
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beta-Lactamases